Tycho™ NT.6 enables scientists to quickly and precisely verify their protein quality, purity, concentration, functionality and similarity — in a single and short experiment. The technology behind NanoTemper Technologies instrument relies on a real time monitoring of any changes in the intrinsic protein fluorescence, detected at both 350 nm and 330 nm, from tryptophan and tyrosine residues in the measured sample. This simple and trustworthy approach verifies protein characteristics by looking at the structural integrity using samples intrinsic signal. As a thermal ramp is applied, the changes in the intrinsic fluorescence are measured constantly and the results indicate transitions in the folding state of a given protein sample. Tycho NT.6 allows testing and comparing the relative quality and stability of a proteins during any step of a purification or detailed characterization workflow. Results are generated in three minutes which means better and faster decisions can be made to potentially take or stop before any following experimental steps. The influences of buffer formulation and/or storage conditions on relative stability and similarity of either freshly prepared or batch-to batch preparations are swiftly determined. Tycho NT.6 automatically generates thermal unfolding profiles, identifies inflection temperatures (Ti), analyzes interactions effects on relative stability and monitors fluorescence sample brightness providing keen insight on sample quality and possible functionality.