National Cryo-EM Facility

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Soon in the National Cryo-EM Center based in Synchrotron SOLARIS there will be Krios™ G3i Cryo-TEM – Transmission Electron Microscope available for the first users. We hope to join the call starting in September and ending on October 1st, 2019. Follow our website and SOLARIS webiste for details.

Krios G3i Cryo-TEM

State-of-the-art
Transmission Electron Microscope

  • 300kV
  • Ultra-stable
  • Semi-automated
  • Grid AutoLoader
  • Three-condenser lens system
  • Gatan K3 BioQuantum
  • BioQuantum Energy filter and embedding
  • Thermo Falcon3EC
  • Ceta 16M
  • Phaseplate

DETECTORS

Gatan K3 BioQuantum

with energy filter and BioQuantum Embedding

  • field of view 5760 x 4092 px with max image size 11520 x 8184 px Super-resolution
  • GPU-based motion correction
  • >1500 full fps; >75 fps to storage
  • DQE for 300kV (200kV): Nq peak >0.83(0.87); 0.5Nq >0.53(0.53)

Falcon 3EC

  • 4096 × 4096 px with 14 µm pixel size
  • 40 fps to storage
  • drift correction
  • DQE at 0.5Nq >0.7

Ceta 16M Camera
300 kV

  • 4096 × 4096 px CMOS sensor with 14 µm pixel size
  • up to 25 fps (512x512px)
  • DQE at 0.5Nq >0.09

We help users with sample preparation and storage. Please visit our services website or contact us. 

State-of-art cryo transmission electron microscope FEI Titan Krios 3Gi enables researchers to look at the macromolecules almost in their natural environment. Frozen hydrated specimens of e.g. proteins make high resolution structural analysis possible. Acquiring SPA datasets gives an opportunity to get molecule structure with a resolution of about 2Å, which means no more ‘blobology’ and very good insight in macromolecules internals. The use of direct electron detectors pushes scientific work output even further. Very fast data acquisition, with framerate up to 75 fps, drift correction and image averaging is now possible during the measurement itself. Such procedure drastically reduces the disk size of datasets and allows to obtains more images at the same period of time comparing to work without direct detectors. Well known disadvantage of vitrificated samples imaging i.e. low contrast can also be overcome with the use of direct electron detectors and when combined with the usage of phase plate, macromolecular structures can be reconstructed reproducibly with very high, unreachable until now, resolutions and quality. Staying in the subject of time, work efficiency and reproducibility requires a few words about automation to be said. Krios system is equipped with autoloader able to hold and handle twelve samples. Additionally, the use of dedicated software for data acquisition, either for SPA or angle tomography, allows full automation of the collection process.
Michał Rawski, PhD
Microscope Operator

IT Infrastructure and Data Storage Policy

  • user data will be stored for 3 weeks and then automatically deleted
  • we do not provide any processing cluster
  • we recommend using Prometheus - Cyfronet AGH for analysing your data